TABLE 1.
Relative protection of mice against Borrelia infection through immunization with dimeric and monomeric recombinant OspC constructs
| Antigen | Challenge | No. of animals | Dosage (μg) | No. of immunizations | No. infected/total no. | % protection |
|---|---|---|---|---|---|---|
| Summary, experiments with B31 immunization and challenge | ||||||
| Alum only | B31 | 27 | 0 | 4 | 27/27 | 0 |
| Alum + OspC-DB31 | B31 | 63 | 0.1–100 | 4 | 2/60 | 97 |
| Alum + OspC-MB31 | B31 | 45 | 0.1–100 | 4 | 32/39 | 18 |
| MPL only | B31 | 3 | 0 | 3 | 3/3 | 0 |
| MPL + OspC-DB31 | B31 | 6 | 1 | 3 | 0/6 | 100 |
| MPL + OspC-MB31 | B31 | 6 | 1 | 3 | 3/6 | 50 |
| Experiment 1, antigen dosage effects | ||||||
| Alum only | B31 | 6 | 0 | 4 | 6/6 | 0 |
| Alum + OspC-DB31 | B31 | 6 | 100 | 4 | 0/6 | 100 |
| Alum + OspC-DB31 | B31 | 6 | 30 | 4 | 0/6 | 100 |
| Alum + OspC-DB31 | B31 | 6 | 10 | 4 | 0/6 | 100 |
| Alum + OspC-M31 | B31 | 6 | 100 | 4 | 5/6 | 17 |
| Alum + OspC-MB31 | B31 | 6 | 30 | 4 | 5/6 | 17 |
| Alum + OspC-MB31 | B31 | 6 | 10 | 4 | 4/6 | 33 |
| Experiment 2, antigen dosage effects | ||||||
| Alum only | B31 | 6 | 0 | 4 | 6/6 | 0 |
| Alum + OspC-DB31 | B31 | 6 | 10 | 4 | 1/6 | 83 |
| Alum + OspC-DB31 | B31 | 6 | 1 | 4 | 0/6 | 100 |
| Alum + OspC-DB31 | B31 | 6 | 0.1 | 4 | 1/6 | 83 |
| Alum + OspC-MB31 | B31 | 6 | 100 | 4 | 4/6 | 33 |
| Alum + OspC-MB31 | B31 | 3 | 10 | 4 | 2/3 | 33 |
| Alum + OspC-MB31 | B31 | 3 | 1 | 4 | 3/3 | 0 |
| Alum + OspC-MB31 | B31 | 3 | 0.1 | 4 | 3/3 | 0 |
| Experiment 3, no. of immunizations | ||||||
| Alum only | B31 | 3 | 0 | 4 | 3/3 | 0 |
| Alum + OspC-DB31 | B31 | 6 | 1 | 4 | 0/6 | 100 |
| Alum + OspC-DB31 | B31 | 6 | 1 | 3 | 1/6 | 83 |
| Alum + OspC-DB31 | B31 | 6 | 1 | 2 | 2/6 | 67 |
| Alum + OspC-MB31 | B31 | 6 | 1 | 4 | 6/6 | 0 |
| Experiment 4, no. of immunizations | ||||||
| Alum only | B31 | 3 | 0 | 4 | 3/3 | 0 |
| Alum + OspC-DB31 | B31 | 6 | 1 | 4 | 0/6 | 100 |
| Alum + OspC-DB31 | B31 | 6 | 1 | 3 | 1/6 | 83 |
| Alum + OspC-DB31 | B31 | 6 | 1 | 2 | 4/6 | 33 |
| Experiment 5, cross-strain challenge | ||||||
| Alum only | B31 | 3 | 0 | 4 | 3/3 | 0 |
| Alum + OspC-DB31 | B31 | 6 | 1 | 4 | 0/6 | 100 |
| Alum only | N40 | 6 | 0 | 4 | 6/6 | 0 |
| Alum + OspC-DB31 | N40 | 6 | 1 | 4 | 6/6 | 0 |
| Alum only | 297 | 6 | 0 | 4 | 6/6 | 0 |
| Alum + OspC-DB31 | 297 | 6 | 1 | 4 | 6/6 | 0 |
| Experiment 6, different lots of recombinant proteins | ||||||
| Alum only | B31 | 3 | 1 | 4 | 3/3 | 0 |
| Alum + OspC-DB31 (lot 1) | B31 | 3 | 1 | 4 | 0/3 | 100 |
| Alum + OspC-DB31 (lot 2) | B31 | 3 | 1 | 4 | 0/3 | 100 |
| Alum + OspC-DB31 (lot 3) | B31 | 3 | 1 | 4 | 0/3 | 100 |
| Alum + OspC-MB31 (lot 2) | B31 | 3 | 1 | 4 | 3/3 | 0 |
| Alum + OspC-MB31 (lot 3) | B31 | 3 | 1 | 4 | 1/3 | 67 |
| Experiment 7, OspC from different strains | ||||||
| Alum only | 297 | 3 | 0 | 4 | 3/3 | 0 |
| Alum + OspC-D297 | 297 | 6 | 1 | 4 | 0/6 | 100 |
| Alum + OspC-M297 | 297 | 6 | 1 | 4 | 5/6 | 17 |
| Alum only | N40 | 3 | 0 | 4 | 3/3 | 0 |
| Alum + OspC-DN40 | N40 | 6 | 1 | 4 | 0/6 | 100 |
| Alum + OspC-MN40 | N40 | 6 | 1 | 4 | 0/6 | 100 |
| Experiment 8, Cross-strain challenge, MPL adjuvant, and reduced dimer immunization | ||||||
| MPL only | B31 | 3 | 1 | 3 | 3/3 | 0 |
| MPL + OspC-MB31 | B31 | 6 | 1 | 3 | 3/6 | 50 |
| MPL + OspC-DB31 | B31 | 6 | 1 | 3 | 0/6 | 100 |
| MPL + OspC-DB31 | B31 | 6 | 1 | 2 | 2/6 | 67 |
| MPL + OspC-DN40 | B31 | 3 | 1 | 3 | 3/3 | 0 |
| None | 297 | 3 | 1 | 3 | 3/3 | 0 |
| MPL + OspC-DN40 | 297 | 3 | 1 | 3 | 2/3 | 33 |
| MPL only | N40 | 3 | 1 | 3 | 3/3 | 0 |
| MPL + OspC-DN40 | N40 | 3 | 1 | 3 | 0/3 | 100 |