Figure 5.
(A) Disulfide cross-linking experiment with 1 μM R69Cys in the presence of 0.5 μM OR1. The figure shows the TIC from RP-HPLC of aliquots taken at increasing reaction times. The compound with retention time RT = 16.7 min is monomeric R69Cys, the compound with RT = 17.7 min is R69Cys disulfide dimer. (B) Disulfide cross-linking experiment with 1 μM R69Cys in the absence or in the presence of specific and non-specific oligonucleotides at different protein–DNA ratios. The points are the average of three independent experiments and represent the fraction of dimer formed (determined dimer concentration divided by half the initial monomer concentration) at subsequent intervals.