Table 2. Course of the cordycepin aptamer selection.
Round | RNA quantity (nmol) | [RNA] (μM) | Pre-selection | Percentage of input RNA eluted with | |
---|---|---|---|---|---|
ATP | Cordycepin | ||||
1 | 11 | 2 | Yes | — | <0.5 |
2 | 2.5 | 1 | Yes | — | 0.5 |
3 | 0.35 | 0.2 | Yes | 1.4 | <0.1 |
4 | 0.35 | 0.2 | Yes | — | 19.5 |
5 | 0.2 | 0.1 | Yes | 19 | 1 |
6a | 0.2 | 0.1 | Yes | <0.1 |
aOn round 6, cordycepin agarose was diluted with Tris-derivatized sepharose to a final concentration of 5 pmol cordycepin/ml sepharose. An aliquot of 200 μl of cordycepin sepharose were used.