Figure 2. SDS-PAGE and gel filtration analyses of recombinant POD in purified state.

(A) A purified sample treated with 2% SDS plus 2% β-mercaptoethanol and boiling (lane 2) and loaded onto 10% polyacrylamide gel. Lane 1 represents the standard proteins whose molecular weights were indicated on the left side of the gel. (B) The molecular weight of rPOD in the solution was determined by Sephacryl S-200 gel filtration using standard proteins as mentioned in Methods.