Table 1. Specific activity and Fe content of the recombinant POD.

	Specific activity (μmol/min/mg protein)	Ratio of Fe atoms by subunit (mol/mol)
Purified	0.087	0.03
Reactivated	3.02	1.1 (estimated)
Dialyzed	1.52 ± 0.24	0.54 ± 0.01

Specific activity and Fe content of the rPOD in the purified, reactivated, and dialyzed states were determined. Reactivation of the purified rPOD was carried out by adding 100 μM FeSO₄ to the sample as described in Materials and Methods. After dialyzation of the reactivated preparation to remove unbound Fe^(II), Fe concentrations of rPOD was determined by three individual measurements. The molecular weight value of the subunit of 30,000 was used to estimate the ratio of Fe atoms by subunit (mol/mol). The result is indicated with mean ± standard error (S.E.).