. 2004 Sep 30;32(17):5126–5133. doi: 10.1093/nar/gkh841

Table 1. The thermodynamic parameters for the four hairpin pairs forming a kissing complex are shown. The T_m is shown for a total RNA concentration of 10⁻⁵ M.

Complementary loop sequence	Na⁺ conc. (mM)	T_m(°C) (for 10⁻⁵ M RNA conc.)	ΔT_m (°C)	−ΔH_app (kcal/mol)	−ΔS_app (cal/mol)	−ΔG_37app (kcal/mol)	−ΔΔG₃₇^a (kcal/mol)	−ΔΔG₃₇^b (kcal/mol)
CCUGCCGGACGG	1000	67.3 ± 0.4	19.0	75.7 ± 1.2	196.6 ± 0.5	14.72	−4.62	−1.12
CCGACCGGCUGG	1000	64.7 ± 0.4	19.8	68.0 ± 2.2	175.8 ± 0.9	13.48	−3.98	−0.48
GAGAGGCUCUCC	1000	57.0 ± 0.3	19.5	71.7 ± 1.6	191.6 ± 0.6	12.28	−4.08	−0.58
CUAAACGAUUUG	1000	32.0 ± 0.3	30.9	35.3 ± 2.2	89.8 ± 1.8	7.45	−4.15	−0.65
						mean:	−4.21	−0.71

The ΔT_m shows the differences between the measured melting temperatures and the predicted T_m for regular RNA duplexes with identical sequence and without dangling adenines. The two columns on the right show the differences in free energy compared to duplexes without dangling adenines (a) and with dangling adenines (b). In both cases, the free energy of the kissing complex is lower than for the corresponding RNA duplexes, indicating that at 1 M Na⁺, the kissing complex is more stable.

Table 1. The thermodynamic parameters for the four hairpin pairs forming a kissing complex are shown. The Tm is shown for a total RNA concentration of 10−5 M.

Table 1. The thermodynamic parameters for the four hairpin pairs forming a kissing complex are shown. The T_m is shown for a total RNA concentration of 10⁻⁵ M.