Fig. 1.

LMP1 enhances tumor growth of TC-1 cells in vivo. a Western blot analysis of LMP1 expression in TC-1 cells. TC-1 cells were transfected with either pLenti6/V5-DEST (Mock) or LMP1/pLenti6/V5-DEST (LMP1) plasmid. Stable Mock or LMP1-expressing cells were selected with blasticidin (3 μg/ml) for 2 weeks before further experiments. β-actin was an internal control. b Immunohistochemistry of LMP1. Paraffin embedded Mock or LMP1 xenografts were stained with anti-LMP1 antibody. Scale bar, 50 μm. c Effects of LMP1 on tumor growth in vivo. C57BL6/J mice were subcutaneously injected with 1 × 10⁵ of ether Mock or LMP1-expressing TC-1 cells (n = 3) and tumor sizes were measured every 5 days. *, P < 0.05. d Images of Mock and LMP1 xenografts. Scale bar, 1 cm