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. 2017 Jan 5;17:18. doi: 10.1186/s12885-016-3027-1

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Vaccination of LMP1/pcDNA3.1 suppresses LMP1-expressing tumor growth in vivo. a LMP1/pcDNA3.1 vaccine construct. Restriction sites are as indicated. CRT, calreticulin. b Effect of vaccination on LMP1-expressing tumor growth in vivo. Upper panel, schedule of tumor injection and vaccination. Lower panel, tumor growth curves. *, P < 0.05. C57BL/6 J mice were subcutaneously injected with 1 × 10⁵ LMP1-expressing TC-l cells (D0). Vaccination with either empty pcDNA3.1 (control) or LMP1/pcDNA3.1 (vaccine) (n = 3) were given weekly on the 7th day after tumor injection for 3 consecutive weeks. Tumor volumes were measured every 3 days and the mice were sacrificed on the fourth week