Fig. 3.
Vaccination of LMP1/pcDNA3.1 suppresses LMP1-expressing tumor metastasis in vivo. a Schedule of tumor injection and vaccination with or without cisplatin. C57BL/6 J mice were intravenously injected with 1 × 105 LMP1-expressing TC-l cells (D0). On the 7th day after tumor injection, 2 μg of either empty vector (control) or LMP1/pcDNA3.1 (vaccine) was delivered to abdominal area weekly for 3 consecutive weeks. For evaluation of combination therapy, cisplatin (10 mg/kg) was given intraperitoneally at the same time of vaccination. Mice were sacrificed on the fourth week after tumor injection. b Number of lung nodules in each group with different treatments (n = 4). *, P < 0.05. **, P < 0.01. c Representative images of lungs