Fig. 6.

LMP1/pcDNA3.1 vaccination increases LMP1-specific T lymphocyte activities ex vivo. a INF-γ ELISPOT assay for evaluation of spot forming cells (SFCs) from control or vaccine groups with or without LMP1 activation. C57BL/6 J mice were vaccinated weekly with either empty vector (control) or LMP1/pcDNA3.1 (vaccine) (n = 5) for 3 weeks. Subcutaneous injection of 1 × 10⁶ LMP1-expressing TC-1 cells was given 3 weeks after the last dose of vaccination. Mice were sacrificed 2 weeks after tumor injection and splenocytes were harvested for ELISPOT assay. Splenocytes were incubated with or without LMP1 peptides for 24 h as indicated. **, P < 0.01. b Representative images of SFCs incubated with LMP1 peptides from control or vaccine group