Fig. 4.

The effects of 5-aza-CdR and DZNeP on MEG3 expression. a MEG3 promoter was hypermethylation in HeLa and CaSki cells (0 μmol/L). MEG3 promoter could be demethylation by different concentrations of 5-aza-CdR (5 and 10 μmol/L) in HeLa and CaSki cells. b MEG3 was re-expressed in the HeLa and CaSki cells treated with different concentrations of 5-aza-CdR (5 and 10 μmol/L), compared to cells of the control group (0 μmol/L 5-aza-CdR). c EZH2 protein levels in cells treated with DZNep (0, 5 μmol/L) were examined by western blot d MEG3 expression was analyzed by RT-qPCR in cells treated with DZNep (0, 1, 5 μmol/L) for 5 days and there was no significant statistical difference among the groups