Fig. 2.

CCL8 mRNA and protein levels in lung tissue-derived fibroblasts from 14 IPF patients and 10 controls. (a) RT-PCR, (b) densitometry of the CCL8 RT-PCR band intensity normalized to that of β-actin, (c) real-time PCR, and (d) correlations of the CCL8 mRNA levels of 12 subjects determined by the transcriptome chip with those by real-time PCR. e, f CCL8 protein level of Culture media and cell lysate, and (g) correlations of the CCL8 protein levels and CCL8 mRNA levels of 24 subjects determined by the ELISA with those by real-time PCR. The data were presented as median values with 25 and 75% quartiles