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. 2004 Oct 4;32(17):5163–5173. doi: 10.1093/nar/gkh847

Figure 6.

Figure 6

Inversion of the triplex orientation affects the reversal of the Top1-mediated DNA cleavage. (A) Sequence of the 30mer (III) duplex DNA showing the reverse orientation of the TFO compared with the 30mer (II) (see Figure 1). (B) Similar Top1 reactions as in Figures 2B, 4B and 5B were carried out using the 30mer (III) oligonucleotide. (C) Using the same oligonucleotides as in panel B, salt reversal reactions (as in Figures 3, 4D, 4E and 5C) were carried out at 25°C for the indicated times (min). Time 0 refers to the samples taken immediately before the NaCl addition. (D and E) Graphical representation of the data shown in panels B and C, respectively. The dashed line in panel D corresponds to the Top1-mediated DNA cleavage in the absence of CPT or TFO. Computed kinetic constants are summarized in Table 2.