Figure 2.
Effects of the inducible expression of polβ on HR. (A) Examination by western blotting of the induction of polβ in the βIND cells after removing doxycycline from the culture medium during 3 days. Immunodetection was performed with an antibody directed to the myc epitope of tagged polβ expressed with the Tet-Off system. Actin was used as control for equal loading. (B) For recombination measurement, the data are expressed as the frequency of neo resistant or neo and hyg resistant colonies observed for 106 viable cells plated. The data are the results of two independent experiments and are expressed as the mean values ± SD.