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. 2016 Jul 4;25(17):3654–3675. doi: 10.1093/hmg/ddw212

Figure 8.

Figure 8.

Q175F and Q175FDN mice display reduced striatal DARPP-32 and mutant Htt inclusions. Striatal DARPP-32 immunoreactivity and cortical EM48 positive Htt inclusions were assessed at 3, 6, 9, and 12 months of age in Q175FDN mice and at 12 months of age in Q175F mice. (A) Representative micrographs of DARPP-32 stained striata at 12 months of age. (B) Integrated optical density of striatal DARPP-32 immunoreactivity shows a decrease in Q175FDN HOM mice from 3 months of age and in HET and, to a greater degree, HOM mice of both lines at 12 months of age. (C) Q175FDN HET, and to a greater degree, HOM mice display EM48 positive cortical muHtt inclusions from as early as 3 months of age. Cortical muHtt inclusions were also observed at 12 months of age in Q175F HET and, to a greater degree, HOM mice. (D) Low magnification micrographs in Q175FDN HOM mice show that inclusion deposition is layer specific in younger mice, but more uniform throughout the cortex in older mice, indicating that deposition occurs earliest in specific cortical layers. *=different from WT same age/line, ns = not significant, **= P < 0.01, ***= P < 0.001.