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. 2016 Jul 4;25(17):3689–3698. doi: 10.1093/hmg/ddw215

Figure 3.

Figure 3.

Compound 1a does not reduce RNA:DNA hybrid formation. The RNA:DNA hybrid was immunoprecipitated with S9.6 antibody from untreated control (GM06865 and GM06895), UFM (GM06897) and AZA treated FXS patient cells at day 6 (GM04025 and GM07294) (A) and AZA treated FXS patient cells (GM04025 and GM07294) with and without 1a treatment at day 6 (B). DNA:RNA immunoprecipitation (DRIP) signal shown is an average of two independent experiments. The error bars represent the standard deviation. The γ-actin locus is a positive control region and ZNF554 is a negative control region for RNA:DNA hybrid.