Table 2.
Short Chain Fatty Acid Analysis at the 2 Different Retention Times for Each Reactor
| Conc (mM) | P48 | T48 | D48 | P96 | T96 | D96 |
|---|---|---|---|---|---|---|
| Acetate | 50.2 ± 1.7 | 19.7 ± 1.5 | 26.1 ± 2.2 | 60.8 ± 1.5a | 19.9 ± 3.5 | 20.3 ± 1.4a |
| Propionate | 19.9 ± 0.6 | 5.4 ± 1.7 | 8.7 ± 1.1 | 22.7 ± 1.3a | 8.6 ± 0.9a | 8.6 ± 0.4 |
| Butyrate | 14.1 ± 1.5 | 3.4 ± 0.1 | 9.0 ± 1.0 | 17.1 ± 0.4a | 9.5 ± 0.9a | 11.6 ± 2.5 |
| Total APB | 84.2 ± 0.7 | 28.5 ± 3.2 | 43.8 ± 4.2 | 100.6 ± 3.1a | 38.0 ± 4.8a | 40.5 ± 4.3 |
| Valerate | 2.9 ± 0.1 | 0.8 ± 0.0 | 3.3 ± 0.6 | 2.8 ± 0.1 | 1.3 ± 0.2a | 1.7 ± 0.3a |
| Caproate | 2.1 ± 0.1 | 0.7 ± 0.1 | 2.3 ± 0.1 | 2.0 ± 0.0 | 0.9 ± 0.0a | 1.8 ± 0.2a |
| Heptanoate | 0.7 ± 0.1 | 0.7 ± 0.1 | 1.6 ± 0.1 | 0.7 ± 0.0 | 0.7 ± 0.0 | 1.7 ± 0.3 |
| Isobutyrate | 4.9 ± 0.3 | 1.3 ± 0.1 | 2.7 ± 0.4 | 4.7 ± 0.1 | 3.4 ± 0.0a | 2.8 ± 0.6 |
| Isovalerate | 4.2 ± 0.5 | 1.0 ± 0.1 | 2.5 ± 0.2 | 3.7 ± 0.1 | 3.5 ± 1.0a | 6.1 ± 1.0a |
| Isocaproate | 2.5 ± 0.0 | 1.0 ± 0.1 | 2.3 ± 0.1 | 2.6 ± 0.1 | 1.4 ± 0.1a | 1.3 ± 0.1a |
| Total SCFA | 101.5 ± 0.4 | 33.8 ± 3.7 | 58.5 ± 5.7 | 117.0 ± 3.4a | 49.1 ± 5.9a | 55.7 ± 6.3 |
P48, T48, and D48 indicate respectively the P (proximal), T (transversal), and D (distal) reactor simulating a 48-hour transit while P96, T96, and D96 indicate respectively the P (proximal), T (transversal), and D (distal) reactor simulating an 96-hour transit. “Conc” stands for the concentration of short chain fatty acids (SCFAs) expressed in mM. Values reported in the table are corrected values (eg, the concentration in the reactor T48 corresponds to the concentration measured in the reactor to which the concentration of the reactor P48 was subtracted). “Total APB” indicates the total concentration of the 3 main SCFAs acetate, propionate and butyrate.
Indicates SCFA concentrations at the slow retention time which are significantly different (P < 0.05) to the values obtained in the same reactor at the normal simulated transit time.