FIG. 1.

Overview of the human adipogenesis study design. Chemical selection from ToxCast Phase 1 screening consisted of 49 chemicals identified in 3 independent PPARG binding and transactivation assays. 11 “low active” chemicals were selected as experimental negatives based on hit ratios across all ToxCast assays. A primary screen with 60 chemicals was run in a functional adipogenesis assay to identify viability ranges for each chemical, as well as activity for the differentiation endpoint. Assay hits were then evaluated across 4 additional orthologous assays spanning 7 endpoints that are specific for molecular and phenotypic features of human adipocytes. All data was analyzed in concentration-response format using a universal assay platform. Chemical hits identified in each assay were scored and classified according to hit frequency, efficacy, and potency.