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Mn-dependent increase in mitochondrial H2O2 production measured by MitoPY1. (A) Live cell mitochondrial H2O2 production was visualized by fluorescence microscopy using MitoPY1 (green) and bright field. (B) Mean fluorescence intensity for each group was determined and plotted as relative % increase compared with control group (0 μM Mn treatment). The data are shown as mean ± SE (n ≥ 5). *P < .05 versus control group.
