Table 1. FRET and bending parameters of the target DNAs upon binding of GCN4-bZIP dimer at 20°C in 100 mM KCl, 10 mM potassium phosphate (pH 6.0).
U-shaped DNA | FE | FRET efficiency (E) | Rda (Å) | Bend angle (°) |
---|---|---|---|---|
Free AP-1 DNA | 0.1534 ± 0.0006 | 0.1163 ± 0.0005 | 70.1 ± 0.5 | 11 ± 1 |
Complex:bZIP:AP-1 DNA | 0.1857 ± 0.0007a | 0.1295 ± 0.0006 | 61.8 ± 1.5 | 20 ± 2 |
Free ATF/CREB DNA | 0.1562 ± 0.0006 | 0.1252 ± 0.0006 | 69.1 ± 0.5 | 12 ± 1 |
Complex:bZIP:ATF/CREB DNA | 0.2140 ± 0.0007a | 0.3100 ± 0.0007 | 57.1 ± 1.5 | 25 ± 2 |
aAsymptotic values of FRET effect.