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. 2004 Sep 30;32(17):5192–5197. doi: 10.1093/nar/gkh854

Table 1. FRET and bending parameters of the target DNAs upon binding of GCN4-bZIP dimer at 20°C in 100 mM KCl, 10 mM potassium phosphate (pH 6.0).

U-shaped DNA FE FRET efficiency (E) Rda (Å) Bend angle (°)
Free AP-1 DNA 0.1534 ± 0.0006 0.1163 ± 0.0005 70.1 ± 0.5 11 ± 1
Complex:bZIP:AP-1 DNA 0.1857 ± 0.0007a 0.1295 ± 0.0006 61.8 ± 1.5 20 ± 2
Free ATF/CREB DNA 0.1562 ± 0.0006 0.1252 ± 0.0006 69.1 ± 0.5 12 ± 1
Complex:bZIP:ATF/CREB DNA 0.2140 ± 0.0007a 0.3100 ± 0.0007 57.1 ± 1.5 25 ± 2

aAsymptotic values of FRET effect.