Figure 1. C3G knock-down enhances migration of MEFs through a mechanism dependent on p38α.

Wound healing assay. MEFs (wt and p38α−/−, with (shC3G) or without C3G knock-down or expression of DNRap1 (DNRap1)) were maintained in the absence of serum and allowed to migrate. A. Representative images from phase contrast microscope after 0 and 8h of migration. B. and C. Histograms show the mean ± S.E.M. of the percentage of wound closure (n = 4). ⁺⁺p < 0.01 and ⁺⁺⁺p < 0.001, versus wt; **p< 0.01; ***p < 0.001, compared as indicated. C. Effect of p38α/β inhibition with the chemical inhibitor, SB203580 (10μM), on cell migration.