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. 2016 Jun 9;7(29):45249–45262. doi: 10.18632/oncotarget.9932

Figure 2. Pyrvinium pamoate potentiates genotoxic agents in vitro and in vivo.

Figure 2

(A) The synergy of pyrvinium pamoate with five different genotoxic agents. Combinatorial index values at 50% effective dose (ED50) or 75% effective dose (ED75) were calculated using CalcuSyn software. (B) HuR is required for the synergy of pyrvinium pamoate with chemotherapeutic agents. Cell viability was determined, and a two-tailed unpaired t-test was performed. (C) Addition of pyrvinium pamoate enhances chemotherapeutic efficacy in spheroid growth assays. 5637 cells were exposed to 320 nmol/L of pyrvinium pamoate, 6.8 μmol/L of cisplatin or the combined treatment at their half concentrations for 15 days. Left, representative spheroids; right, colony number. (D and E) Tumor volume of two primary bladder cancer xenograft models. Tumor volumes were recorded every other day during treatments (n = 8–12 each group). (F and G) Tumor weight in mice. Solid tumor weight was measured on day 28 (n = 8–12 each group). Statistical comparisons were performed by One-way ANOVA analysis. Columns and dots, mean; bars, standard deviation. ns, not significant; *P < 0.05; **P < 0.01; ***P < 0.001. PP, pyrvinium pamoate; CDDP, cisplatin; Dox, doxorubicin; wt, wild-type; K.O., knockout.