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. 2016 May 19;7(29):45263–45274. doi: 10.18632/oncotarget.9481

Figure 2. MLN4924 reduces human OS cell viability.

Figure 2

a. Human OS cell lines and normal osteoblasts were treated with serial dilutions of MLN4924 for 1 or 4 days and cell viability was determined using MTT assays. Representative inhibitory curves from three independent experiments are shown for each cell line. b, c. MG-63 cells were seeded into 60 mm × 15 mm petri dishes at 3,000 cells per dish in triplicate and treated with MLN4924 for 12 days, followed by 0.01% (w/v) crystal violet staining and colony counting. (b) Representative images of three independent experiments are shown for colony formation. (c) Graph of the relative number of colonies formed (the results of three independent experiments, expressed as mean ± SEM). (**p<0.01).