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. 2016 May 26;7(29):45331–45351. doi: 10.18632/oncotarget.9644

Figure 12. FOXO1 and acetylation of FOXO1 is associated with Aldo-induced podocyte autophagy in vivo.

Figure 12

A. Representative images demonstrating the expression and localization of p-mTOR (green), nestin (red), and DAPI (blue) by indirect immunofluorescent staining in frozen sections of kidneys from rats in each group, as indicated. Scale bar=50 μm.B, C. Western blot analysis revealed the expression of p-mTOR, FOXO1, Ac-FOXO1, Atg7, Rab7 and β-actin proteins from isolated glomeruli from control and Aldo-treated rat kidneys. D. Co-immunoprecipitation of Atg7 and Ac-FOXO1 in glomeruli. Glomeruli were isolated from the fresh kidney tissue in two groups, as indicated. Then, the tissue lysate was extracted for co-immunoprecipitation with anti- Ac-FOXO1 followed by probing with anti-acetylated Atg7. Results (means±SEM) of 3 series of experiments. #P<0.05 vs. normal control.