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. 2016 May 26;7(29):45331–45351. doi: 10.18632/oncotarget.9644

Figure 2. Aldo-induced autophagy flux in cultured podocytes.

Figure 2

A. Western blot analysis revealed the expression of LC3II/LC3I, Atg7, p62, CHOP, Nephrin, Podocin, and β-actin proteins in podocytes after treatment without or with 10-7 M Aldo for various time periods as indicated. B. Graphical presentation indicates the relative abundance levels of LC3II/LC3I, Atg7, and p62 after normalization with β-actin. C. Immunofluorescence staining for LC3 in podocytes after various treatments, as indicated. The yellow arrow indicates nuclear FOXO1, and the white arrow indicates cytoplasmic FOXO1. Scale bar=25 μm. D. Western blot analysis revealed the expression of LC3II/LC3I, p62, and β-actin proteins in podocytes after various treatments, as indicated. E. Graphical presentation indicates the relative abundance levels of LC3II/LC3I and p62 after normalization with β-actin. Results (means±SEM) of 3 series of experiments. #P<0.05 vs. normal control, *P<0.05 vs. Aldo alone.