Table 1. PRAME protein expression vs. molecular and clinical parameters in EOC.
| PRAME mRNA/18S rRNA (RT-qPCR) | PRAME methylation (pyro) | |||
|---|---|---|---|---|
| PRAME protein (IHC H-Score1) | 0.287 (Spearman) p = 0.033 N = 55 |
−0.062 (Spearman) p = 0.686 N = 45 |
||
| ALL EOC (N = 244) | Low H-Score2N = 122 | High H-Score N = 122 | P-value | |
| Age | 62.5 | 64.0 | 0.390 | |
| Primary | Multiple Ovary Primary Peritoneal Fallopian Tube |
1 89 27 1 |
4 99 15 4 |
0.118 |
| Stage | IA, IB, IC, IIC, IIIB IIIC, IV |
25 94 |
25 96 |
1.000 |
| Grade | 1, 2 3, 4 |
21 95 |
27 100 |
0.439 |
| Histology | Serous Clear Cell Endometrioid Mucinous Other |
81 8 7 9 17 |
90 4 6 3 19 |
0.288 |
| Debulking | Optimal Sub |
48 8 |
50 16 |
0.250 |
| Response to Primary Treatment | Complete Otherwise |
62 41 |
62 31 |
0.429 |
| Platinum Sensitivity | Sensitive Resistant/Refractory |
52 30 |
51 31 |
1.000 |
|
PFS OS |
Median Months (95%CI) | 18.5 (13.7–24.3) 39.6 (32.2–52.7) |
15.3 (12.8–17.8) 49.7 (39.8–67.0) |
0.227 0.097 |
| HGSC (N = 160) |
Low H-Score N = 76 |
High H-Score N = 84 |
P-value | |
|
PFS OS |
Median Months (95%CI) | 18.1 (13.7–24.3) 51.1 (41.8–77.1) |
15.3 (12.8–17.9) 41.7 (33.6–53.2) |
0.408 0.227 |
1
H-score (range = 0 to 300) is the extent of cytoplasmic immune staining, and = 3× % strongly staining cytoplasm + 2× % moderately staining cytoplasm + % weakly staining cytoplasm.
2
Low and high H-score groups were split using the median H-score value, 88.3.