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. 2004 Sep 30;32(17):5223–5230. doi: 10.1093/nar/gkh865

Figure 1.

Figure 1

Gel filtration chromatography on the recombinant Sso DNA primase complex. (A) SDS–PAGE analysis of the fractions (2 ml) from the gel filtration chromatography carried out on a Superdex 200 column, as described in Materials and Methods. The elution volume of the Sso DNA primase complex is indicated. The gel was stained with Coomassie blue. Lanes 2–15 were loaded with 20 μl aliquots of fractions 94–107; lane 1 contains marker proteins. (B) The Sso DNA primase complex Stokes radius was estimated to be about 39.2 Å from the reported plot. The gel filtration column was calibrated with the following standard proteins: A, tyroglobulin (Stokes radius: 85.0 Å, Ve: 139 ml); B, ferritin (Stokes radius: 61.0 Å, Ve: 155 ml); C, BSA (Stokes radius: 35.5 Å, Ve: 201 ml); D, ovalbumin (Stokes radius: 30.5 Å, Ve: 213 ml).