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. 2004 Sep 30;32(17):5223–5230. doi: 10.1093/nar/gkh865

Figure 4.

Figure 4

Reaction requirements for the Sso DNA primase activity. (A) The thermal stability of Sso DNA primase was tested by pre-incubating the enzyme for 15 min at the indicated temperatures (NP stands for Not Pre-incubated); then, the enzyme residual activity was assayed on the T-Bubble DNA substrate, as described in Materials and Methods. (B) The influence of pH on the Sso DNA primase activity was studied in the following buffer systems (50 mM final concentration and pH measured at 60°C): MES–NaOH (pH: 5.0 and 5.5); MOPS–NaOH (pH: 5.8); HEPES–NaOH (pH: 6.4 and 7.5); Glycine–NaOH (pH: 8.2), as described in Materials and Methods.