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. 2016 Jun 14;7(29):45462–45478. doi: 10.18632/oncotarget.9990

Figure 5. Western blot analysis indicated that LY294002 (20 μM), YM022 (100 nM), netazepide (100 nM) and Ro-32-0432 (1 μM) all completely reversed while PD98089 (20 μM) partially reversed the reduction in p27 expression caused by 10 nM G17 treatment for 24 h.

Figure 5

(A) A chemically synthesised miR-222 mimic significantly reduced p27 mRNA and protein expression (B, D) whereas a chemically synthesised miR-222 inhibitor partially reversed the reduced p27 mRNA and protein expression that was caused by 10 nM G17 treatment for 24 h (C, E) in AGSGR cells. Statistical significance was determined using one way ANOVA with Tukey post-hoc test or two-way ANOVA with Sidak post-hoc test and P < 0.05 was considered significant. *P < 0.05, **P < 0.001 and ****P < 0.0001.