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Polymerization was determined according to increasing pyrene fluorescence. Berberine (berberine) had a quenching effect on the fluorescence signal of pyrene-actin. A. Berberine had no effect on actin polymerization with the addition of actin polymerization buffer. B. In the presence of 0.5 μM VASP, the inhibitory effect of berberine remained and reduced the final amount of F-actin.
