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. 2016 Jun 13;7(29):45876–45888. doi: 10.18632/oncotarget.9970

Figure 5. Vimentin knockdown induced a cancer stem cell-like phenotype in A2780 cells.

Figure 5

a. Graphical representation of mRNA expression levels of CD133 and ALDH1A1 in A2780-VIM-KN and control cells. b. Western blotting images of the stem cell marker ALDH1A1 in A2780-VIM-KN and control cells. c. FACS analysis of CD133 expression levels in A2780-VIM-KN and control cells. All the results show the means of three independent experiments. Error bars indicate SEM. Data were analyzed using Student's t test. *p < 0.05, **p < 0.01 and ***p < 0.001. d. The morphological image of A2780-control cells grown in ultralow adhesion tissue culture plates for 10 days. e. The morphological image of A2780-VIM-KN cells grown in ultralow adhesion tissue culture plates for 10 days. f. The morphological image of A2780-VIM-KN spheroids re-cultured in the stem cell medium. g. The morphological image of A2780-VIM-KN spheroid re-cultured in the normal medium. h. The morphological image of A2780-VIM-KN cells cultured in the normal medium.