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. 2016 Jun 13;7(29):45889–45900. doi: 10.18632/oncotarget.9969

Figure 2. ODE activates apoptosis in CRC cells.

Figure 2

A panel of established CRC cell lines (HCT-116, Lovo, HT-29 and DLD-1) and three primary human CRC cell lines were treated with or without ODE at applied concentrations, cells were further cultured, cell apoptosis was analyzed by listed assay A-D, G and H. HCT-116 cells were pretreated with Ac-DEVD-CHO (“DVED”), Ac-LEHD-CHO (“LEHD”) or Ac-VAD-CHO (“VAD”) (40 μM each) for 1 h, following by ODE (50 μg/mL) treatment, cell viability E. and cell death F. were tested. “DMSO” stands for 0.1% DMSO. Cleaved-PARP/cleaved-caspase-3 expression (vs. Tubulin) was quantified. Data in this figure were repeated four times, and similar results were obtained. * p < 0.05 vs. “C” group. # p < 0.05 vs. “ODE” only group (E and F).