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. 2016 Jun 13;7(29):45901–45915. doi: 10.18632/oncotarget.9974

Figure 1. NF-YA inactivation in Hela cells triggers activation of apoptotic cell death and p53.

Figure 1

A. Growing curve of Hela cells infected with shCTR and shNF-YA. Time points are indicated. Statistical significance was calculated with independent t-test at 120h (* p < 0.05). B. Percentage of subG1 events determined by Propidium Iodide-FACS analysis of Hela cells 96h post infection with shCTR and shNF-YA lentiviral particles. Statistical significance was calculated with independent t-test (** p < 0.01). C. Percentage of AnnexinV-positive cells 96h post infection with shCTR and shNF-YA. Statistical significance was calculated with independent t-test (** p < 0.01). D. Expression levels of the indicated proteins in Hela whole cell extracts 72h and 96h post-infection with shCTR and shNF-YA. Actin was used as loading control. E. q-RT PCR analysis of the indicated transcripts 72h and 96h post-infection with shCTR and shNF-YA. The housekeeping hRpl19 gene has been used for normalization. Statistical significance is calculated with independent t-test (* p < 0.05; ** p < 0.01). Error bars indicate Standard Error of the Mean (SEM).