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. 2016 Jun 13;7(29):45901–45915. doi: 10.18632/oncotarget.9974

Figure 5. NF-YA inactivation affects the expression of key transcription factors regulating HPV18-URR activity.

Figure 5

A. qRT-PCR analysis of the indicated genes 72h and 96h post-infection with shCTR and shNF-YA. hRpl19 has been used as reference gene. Statistical significance was calculated with independent t-test (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001). Error bars indicate SEM. B. Western blot analysis of the indicated proteins in shCTR and shNF-YA cells 96h post-infection. C. The table indicates the effect of NF-YA loss on gene transcription of the indicated transcription factor (TF), observed by qRT-PCR and gene expression profiling, the binding of NF-Y (ENCODE ChIP-seq data) and the presence (+) or not (−) of canonical NF-Y binding site in TF-promoters (−500bp from the TSS).