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Range of candidate sequencing primers for elucidation including position T1 (e.g. an assembly ambiguity to resolve), with user-specified parameters. Candidates are checked from 3′ to 5′ within the range to minimize the number of primers required. An early candidate could also resolve T2 in the same reaction. U = number of unreadable bases, L = primer length, R = expected sequence read length, M = minimum number of bases to elucidate after T1.
