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. 2016 Jun 14;7(29):45916–45925. doi: 10.18632/oncotarget.10001

Figure 2. KIT mutant cells migrate faster on human dermis than wild type human melanocytes and BRAF mutant melanoma cells.

Figure 2

A. Representative picture displaying the generation of human skin reconstructs in vitro: GFP expressing PHMs or melanoma cell lines mixed with human keratinocytes (ratio 1:6) were seeded in a cylinder on one edge of devitalized human dermis. The rest of the graft was covered with human keratinocytes only. B. Schematic drawing of skin reconstructs indicating the location of the representative microscopic pictures: GFP expressing, KIT mutant cells were able to populate a greater area on devitalized human dermis after 14 days of incubation compared to wild type PHMs or BRAF mutant melanoma cells (c,f). C. Statistical analyses of distance migrated over time by GFP expressing cells (n>3; 2-sided student t-test * p<0.05; error bars indicate the standard deviation).