Skip to main content
. 2016 Jun 14;7(29):45959–45975. doi: 10.18632/oncotarget.10009

Figure 1. RON isoforms are highly expressed in human pancreatic cancer specimens.

Figure 1

A. End point PCR of pancreatic cancer cell lines, human pancreatic ductal adenocarcinoma (PDAC), and patient derived xenografts show the products of alternative splicing. Primers flanking regions which are highly spliced and primers specific to the short form were used to resolve RNA isoforms. B. The PCR data summarized for all cell lines and patient derived xenografts. Exon 11 skipping is the most common event and 2 new splice products were discovered – intron 13 insertion and partial 5 and 6 skipping. C. Absolute quantification PCR quantifies RON expression in patient derived xenografts. As RON expression increases there is a corresponding increase in isoform transcript number. D. The percentage RONΔ165 transcripts increased as RON transcripts increased while the percentages of short form RON and P5P6 were similar. Overall in high RON expressing PDXs almost 50% of transcripts can be attributed to 3 of the isoforms. All other RON transcripts corresponds to the total number of RON transcripts minus the combined measured isoform transcripts. This includes the full length and all other not measured isoforms.