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. 2016 Jun 14;7(29):46014–46027. doi: 10.18632/oncotarget.10014

Figure 4. Ikaros promotes SH2B3 but suppresses IL7R transcription.

Figure 4

A. The promoter activity of SH2B3 and IL7R promoters by luciferase reporter assay following transfection with Ikaros or control vector in HEK293 cells. B. Expression of SH2B3 and IL7R in Nalm6 cells transduced with vector containing Ikaros as compared to control vector. C. Comparison of SH2B3 and IL7R expression in Nalm6 cells treated with Ikaros shRNA (siIkaros) or scramble shRNA (siCTL). Gene expression is determined by RT-qPCR using total RNA isolated from the cells transfected with scramble shRNA (siCTL) or Ikaros shRNA (siIkaros) for 2 days. compared with siCTL in C: *p <0.05, **p<0.01 compared to siCTL group.