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. 2016 Jun 14;7(29):46028–46041. doi: 10.18632/oncotarget.10016

Figure 1. WST-8 cell proliferation assay.

Figure 1

A. NB4 cells were treated with increasing doses of ATO with or without fucoidan (20μg/ml) and cell proliferation was assessed after 48 hours. Tumor cell viability significantly decreased when fucoidan was combined with ATO at both low and therapeutic doses. Mean ± SEM values of three replicates are shown. Statistical significance was determined by two-way ANOVA, followed by Bonferroni post-test (***: p<0.001). B. Non-APL Kasumi cell line was co-treated with ATO and fucoidan and cell proliferation was assessed after 96 hours. Neither alone nor combination of agents affect the non-APL cells viability. Mean ± SEM values of three replicates are shown.