Figure 3. Representative cell differentiation assay.

NB4 cells were treated with low concentration of fucoidan alone (5 μg/ml), low dose of ATRA alone (0.5 μM), low dose of ATO alone (0.5 μM) or in combination and incubated for 5 days. Myeloid differentiation was analyzed by flow cytometry using CD11b expression. Representative percentages display CD11b^high expressing population indicative of differentiated cells in each treated group. In plot histograms, green population demonstrates differentiated population (CD11b^high) while red population represents non/low-differentiated cells (CD11b^neg/low).