Figure 9. MA-mediated ER stress involved caspase-3 and caspase-9 in astrocyte cell death.
A-F. SVGA cells were seeded at 6 × 105 cells in a 6-well plate and transfected with various siRNA as mentioned in methods. After 48 hours, the cells were reseeded in 12-well plate and allowed to adhere overnight. Finally, these cells were treated with 500 μM MA for additional 24 hours. The effect of specific siRNA against ATF6 (A), PERK (B), ATF4 (C), IRE1α (D), p65 (E) and CHOP (F) were assessed on the levels of cleaved and pro-caspase-3 and caspase-9 using western blotting. The western blots are representative images of at least 3 independent experiments. The numbers above the blots represent mean intensity of the respective bands.