Figure 2. ARID1A knockdown activates PI3K-AKT signaling in GC cells.

A. Phospho-protein antibody array analysis of SGC-7901 cells with ARID1A silencing. The phospho-proteins of orange were upregulated, while the grey proteins were downregulated. A black arrow indicates activation, whereas a bar at the end of a straight line indicates suppression. The red arrows indicate transcriptional stimulation. B. ARID1A was transiently silenced in four GC cell lines and the expressions of major components of PI3K-AKT pathway were analyzed. C. ARID1A was silenced in Hela cell line with shRNAs. AR, ARID1A. Luc, luciferase gene. D, E. ARID1A was silenced in SGC-7901, HGC-27 and Hela cells with shRNAs and the expression of ARID1A, BRG1, AKT, p-AKT^S473, PTEN, PI3K and PDK1 were analyzed. F. ARID1A was first stably knocked down by a shRNA in HGC-27 and Hela cells, and the expression of ARID1A was further rescued by the ectopic expression of the full-length gene of ARID1A (3.1-AR (fl)).