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. 2016 Jun 15;7(29):46158–46172. doi: 10.18632/oncotarget.10064

Figure 3.

Figure 3

A. The mRNA expression level of the TNF, COX2, MMP9 and CTNNB in cancer tissues with different abundance of B. fragilis, F. nucleatum and F.prausnizii were analyzed by RT-PCR, and the protein of TNF-α, COX-2, MMP-9, β-catenin and NF-κB were analyzed by western blot B. (n = 8 samples per group). Quantitation of the signals C. were analyzed based on the result of western blot. B-actin was used as the endogenous control. P values were calculated with Student's t test. *: statistically significant P<0.05, **: statistically significant P<0.01.