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. 2016 Jun 17;7(29):46371–46383. doi: 10.18632/oncotarget.10131

Figure 2. Glycosylation of ASCT2 is dependent on glucose availability.

Figure 2

Representative immunoblots from HL-60 leukemia cell extracts depicting A. the time course of ASCT2 deglycosylation in response to glucose withdrawal, B. the extent of ASCT2 deglycosylation in the absence of glucose and upon treatment with tunicamycin (TUN) or 2-deoxyglucose (2-DG) and C. the similar shift to lower MW obtained in the presence of 2-DG or PNGase F. Representative immunoblots from K-562 leukemia cell extracts depicting the extent of ASCT2 deglycosylation upon D. TUN or E. 2-DG treatment. These experiments were repeated 2-3 times with similar results; β-actin immunobloting was used as loading control.