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. 2016 Jun 17;7(29):46419–46432. doi: 10.18632/oncotarget.10139

Figure 1. MYO1E deletion does not grossly affect mammary gland development.

Figure 1

A. Expression of MYO1E in the mouse mammary gland at 10 weeks of age in MYO1E wild type (WT) and MYO1E knockout (KO) mice as determined by Western blotting. Tubulin was used as a loading control. Molecular marker positions are indicated at 150 kDa and 50 kDa. Anti-MYO1E antibody labels a distinct band at 127 kDa in MYO1E WT mammary glands, which is absent in MYO1E KO mammary glands. B. the overall appearance of the mammary ducts in MYO1E WT and KO mice at 10 weeks of age using histological stains (Hematoxylin & Eosin and Masson's Trichrome) appears very similar, each with two distinct cell layers. The arrow labels the outer basal cell layer, while the arrowhead labels the inner luminal cell layer. Scale bar, 20 um. C. localization of basal epithelial cell marker (Cytokeratin 14 (CK14)) and luminal epithelial cell marker (Cytokeratin 8 (CK8)) in mammary glands of 5 week old MYO1E WT and KO mice. DAPI staining is shown in blue. The boxed region in the left most panel indicates the area that is enlarged in the panels on the right. Scale bar, 100 um. D. whole mount staining of MYO1E WT and KO mammary tissue at 5, 7 and 10 weeks of age shows no clear differences in mammary gland development in mice deficient for MYO1E.