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. 2016 Jun 27;7(29):46603–46614. doi: 10.18632/oncotarget.10314

Figure 3. Δ9-THC and pioglitazone act through divergent pathways.

Figure 3

A. LDH assay demonstrating the additive protective effect of 5 μM pioglitazone (pio) with increasing concentrations Δ9-THC against 7 mM MPP+ toxicity. B. Western Blot and C. QPCR showing the PPARγ protein and mRNA levels of SH-SY5Y cells infected with lentivirus containing a non-specific scramble sequence and PPARγ shRNA respectively. D. LDH assay showing that the PPARγ shRNA construct prevents protection afforded by 10 μM Δ9-THC against 7mM MPP+ but not E. 5 μM pioglitazone. F. DCFDA oxidative stress assay demonstrating that whereas knock down of PPARγ prevents 10 μM Δ9-THC mediated reduction in oxidative stress, PPARγ knock down does not prevent 5 μM pioglitazone-mediated reduction in oxidative stress. Each bar represents the mean ± SEM from 3 independent experiments (one-way ANOVA with Tukey HSD post-hoc test,*p < 0.05, **p < 0.001, ***p < 0.0001 vs. scramble MPP+; #p < 0.05 vs. shRNA MPP+).