Figure 6. Effects of TRF2 mutant on tumorigenesis in nude mice.
A. Control A375 cells or A375 cells conditionally expressing WT-TRF2 or TRF2S323A were subcutaneously injected into nude mice. Doxycycline was added to the drinking water ten days after injection to induce the transgenes expression. The tumor volume is shown and results are expressed as mean ± SD (t-test statistical analysis is included: * p<0.05; ** p<0.01; *** p<0.001). B. Immunoblots showing the expression of TRF2 and actin (loading control) in tumor extracts prepared at the end of the tumor xenograft experiment.