Simulations of the distance distribution probability for the six spin-labeled pairs in the IF (cyan) and OF (magenta) states performed with MMM2015 with the rotamer library at ambient temperature (dotted lines as in
Figure 1) and with the rotamer library of MMM2013 simulated at 175 K (dashed lines). The experimental distance distributions on nucleotide-free (apo, black solid line in upper plots) and ATP-Vi-Mg (black, solid line in lower plots) states are taken from
Figure 2. Transparent cyan and magenta rectangles outline the range of experimental distances characteristic of the IF and OF conformations, respectively.