Table 1.

ATPase activities in detergent.

DOI: http://dx.doi.org/10.7554/eLife.20236.010

Protein		Nucleotide	Temperature [°C]	ATPase activity [nmol Pi/min/mg protein]	Turnover per transporter [min−1]	% of wildtype
TM287/288	wildtype	ATP - Mg	50	2141 ± 67	284	-
			25	86.1 ± 2.5	11.4	-
		ATP – Mg +1 mM vanadate	50	165 ± 20	21.9	7.71
		ATP – Mg +2.5 mM EDTA	50	<0.1	<0.01	<0.005
		AMP-PNP - Mg	25	<0.1	<0.01	<0.005
	E517QTM288	ATP - Mg	25	0.165 ± 0.015	0.0219	0.192
Spin-labeled TM287/288	350TM287/475TM288	ATP - Mg	50	-	-	193*
	460TM287/363TM288	ATP - Mg	50	-	-	212*
	131TM288/248TM288	ATP - Mg	50	-	-	156*
	231TM287/304TM287	ATP - Mg	50	-	-	146*
	150TM287/295TM288	ATP - Mg	50	-	-	112*
	50TM287/271TM287	ATP - Mg	50	-	-	73*
BmrCD	wildtype	ATP - Mg	25	22.9 ± 0.5	3.24	-
	E592QBmrD	ATP - Mg	25	0.633 ± 0.053	0.0896	2.76
MsbA	wildtype	ATP - Mg	30	135 ± 9	18.4	-
	561MsbA	ATP - Mg	30	122 ± 17	16.6	90.4

* values are given in respect to an internal wildtype control in each measurement.