Fig. 3.

Effects of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on peroxisome proliferator-activated receptor gamma co-activator 1-alpha (PGC-1α) promoter activity in C₂C₁₂ muscle cells. Differentiated cells were transfected with the PGC-1α (−1790/+52 bp)/luc reporter gene and pCMV-β galactosidase and were incubated 1% bovine serum albumin serum-free medium with 0 (control) to 50 μM EPA (A) and DHA (B) for 40 h. Promoter activities measured by luciferase activity were calculated in relative light units (RLU) and normalized to β-galactosidase activity. Values are expressed as mean±SD (n=3) of three independent experiments. *P<0.05, **P<0.01, and ***P<0.001 compared to control.